منابع مشابه
The voltage clamp and cardiac electrophysiology.
• Although voltage clamp methods have been applied experimentally to cardiac tissue for over a decade, this field of study has failed to achieve general acceptance because of limitations of the experimental approach and difficulty in interpreting the results. Yet this technique is valuable within its limitations, and to dismiss it would be to lose a valuable tool. In perspective, most technique...
متن کاملVoltage Clamp Experiments in Striated Muscle Fibers
1. Membrane currents during step depolarizations were determined by a method in which three electrodes were inserted near the end of a fibre in the frog's sartorius muscle. The theoretical basis and limitations of the method are discussed.2. Measurements of the membrane capacity (C(M)) and resting resistance (R(M)) derived from the current during a step change in membrane potential are consiste...
متن کاملVoltage-Clamp Studies on Uterine Smooth Muscle
These studies have developed and tested an experimental approach to the study of membrane ionic conductance mechanisms in strips of uterine smooth muscle. The experimental and theoretical basis for applying the double sucrose-gap technique is described along with the limitations of this system. Nonpropagating membrane action potentials were produced in response to depolarizing current pulses un...
متن کاملAnalysis of cardiac pacemaker potential using a "voltage clamp" technique.
VASSALEE, MARIO. Analysis of card& pacemaker potential using a %ltage clamf3 technique. Am. J. Physiol. 210(6): 1335-1341. I g66.-Diastolic depolarization was studied by means of ccvoItage clamp” technique in short mammalian Purkinje fibers. Clamping the membrane potential at the maximal diastolic value resulted in an inward current rising with a time constant of 3 set in 5.4 mM K, indicating t...
متن کاملVoltage Clamp Experiments on Single Muscle Fibers of Rana pipiens
A voltage clamp for single muscle fibers has been developed. Stability of the system was achieved when an artificial node was created by enclosing a single muscle fiber in a petroleum jelly seal which served as an analogue of the myelin sheath. Typical voltage clamp records were obtained with large inward transient currents followed by a delayed rectification of the outward currents. These curr...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1972
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(72)86177-0